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KMID : 1059520080520010057
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Journal of the Korean Chemical Society
2008 Volume.52 No. 1 p.57 ~ p.65
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Preparation of Protein-coated Cationic Liposomes Containing Doxorubicin and Their Binding Property of Blood Plasma Protein
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Kim Sung-Kyu
Jung Soon-Hwa
Jung Suk-Hyun
Seong Ha-Soo
Chi Sang-Cheol
Cho Sun-Hang
Shin Byung-Cheol
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Abstract
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Liposomes are nanometer or micrometer scale vesicles that can be used as drug delivery carriers. However, plain liposomes are plagued by rapid opsonization, making their circulation time in bloodstream be shortened. In this study, model protein, bovine serum albumin (BSA)-coated liposomes were prepared by coating cationic liposomes with BSA molecules at higher pH than isoelectric point of BSA. The BSA molecules coated on the liposomal surface were denatured by thermal treatment at above 60 oC. While both plain and cationic liposomes had about mean particle diameter of 104±1 nm, BSA-coated cationic liposomes (BCL) had mean particle diameter of 109±1 nm. Encapsulation of model drug, doxorubicin (DOX), in liposomes were carried out by using remote loading method and the loading efficiency of DOX to liposomes was about 90%. The mean particle diameter of BCL did not increase in blood plasma and adsorption of plasma protein was much less than plain or cationic liposomes. These results suggest that BCL can be used as a long-circulating liposomes in bloodstream.
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KEYWORD
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Liposome, Stability, Albumin, Protein binding, Doxorubicin
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